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Cell culture for ARPE-19 cells ARPE-19 cells were cultured in a T-75 cell culture flask in DMEM:F12 media supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin according to ATCC guidelines and as previously described [24]

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The preference of PAGL formation may be explained by the fact that both glutamine- and glycine-N phenylacetyltransferase are present in the dog, with the latter having a higher activity, consequently favoring PAGL formation over that of PAG

Proc Natl Acad Sci USA 101 : 81748179

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