Lysis of cells was performed with a homogenizer (Precellys Evolution) for six cycles of 20 s each at 6500 rev
CARS2 mutant lacking the persulfide synthesis activity did not rescue the decreased mitochondrial membrane potential of CASR2-deficient cells, whereas CARS2 mutant lacking cysteinyl t-RNA synthesis activity did
Galluzzi, L., Buqu, A., Kepp, O., Zitvogel, L
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Storage Lyophilizate (dry powder before reconstitution) 2 to 3 years at 20 C (freezer) 6 to 12 months at 2 to 8 C (refrigerator) Up to 30 days at room temperature (up to 25 C), protect from light and moisture After reconstitution (peptide in solution with bacteriostatic water) Up to 28 days at 2 to 8 C , protected from light After this period degradation products (= broken fragments of the molecule, which do not function) can rise significantly Sterile water without preservative shortens stability to 7 to 10 days Practical storage rules Allow the vial to warm to room temperature (15 to 20 min) before opening
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