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Health 1, 900913
Of note, the commercial Iscoves Modified Dulbeccos Medium (IMDM) and Serum Free Expansion Medium (SFEM), which were used as the basal medium for erythroblast cultures, contain a supraphysiological concentration of cystine at 292 M as compared to physiological plasma concentration of 4075 M cysteine in SCD patients, which decreased further to 3060 M during vaso-occlusive pain episodes 36
We then evaluated whether LCN2 ablation affects antioxidant defenses that counteract ferroptosis, focusing on the cystine/glutamate antiporter System Xc (encoded by Slc7a11/SLC7A11/xCT) and glutathione peroxidase (GPX4), which together mitigates oxidative stress by promoting cystine import and glutathione synthesis [11, 30]
These statements have not been evaluated by the FDA and are not intended to diagnose, treat, cure, or prevent any disease
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