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The shorter half-life also means that IGF-1 DES needs more frequent use to keep steady-state levels, which can be advantageous or disadvantageous depending on the research protocol

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Dividing dose by vial size before calculating concentration is the most common error

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PaSTRy Solutions of EV-F3 and additive at either 100 or 1000 molar excesses (corresponding to 0.1 mM or 1 mM final concentration) were aliquoted into a 96-well PCR plate containing SYTO9 dye (Thermo Fisher Scientific) in triplicate with 50 mM HEPES, pH 8.0, 200 mM NaCl buffer, before centrifugation for 3 min at 4 C