The thiol group derivatization The presented assay is based on RP-HPLC technique coupled with UV detection
Analysis of Gene Expression by Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) By using Qiagen tissue extraction kit (Qiagen, Germantown, MD, USA) and following the manufacturers instructions, total RNA was isolated
Desalting may be vital to remove remnants of other reagents used in cell preparation, like phenol red that has the capacity to bind and interact with some types of purification media
14 GST and GPX activity were measured using a GST Activity Assay Kit (Abcam, Shanghai, China) and Glutathione Peroxidase Cellular Activity Assay Kit (Sigma), respectively
These results collectively demonstrated the size-switchable and on-demand release behavior of the P-Mn/TI@mHSA
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