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doi: 10.1074/jbc.273.1.329

After washing three times using 1x PTW/0.5% BSA solution for at least 30 min (10-min interval), the dissected gonads were stained with DAPI solution (100 ng/mL) for 10 min at room temperature and were then washed with 1x PTW/0.5% BSA solution three times

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10.3389/fphys.2015.00187 77 SpillerF.CostaC.SoutoF

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For this recipe, I opted to combine THD ascorbate with vitamin E and carotenoid-rich annatto