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Firstly all three strains, the GDH2 and GDH3 deletion mutants and the wild-type strain, grown in MM medium were compared to investigate the metabolic differences between mutant and wild-type strains during the morphological transition ( gdh2 -MM vs WT-MM, gdh3 -MM vs WT-MM)

Then, the skin is carefully aligned and sutured shut to ensure the stitches are hidden as much as possible