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The bacterial tryptophanase pathway yields indole which can be delivered to the liver via the enterohepatic circulation where it is metabolized to indoxyl and then indoxyl sulfate by the CYP2E1 and SULT1A1 encoded enzymes, respectively

This makes it easier to get rid of them through urine or bile

This cascade of events can lead to neurotoxicity, synaptic malfunction, and, in severe circumstances, neuronal death (Haruwaka et al., 2019)

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New York: CRC Press

For a subcutaneous injection, which goes into the fatty tissue just beneath the skin, a 45-degree angle is best