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An apparent paradox is that whereas GLP-1 increases glucose-stimulated insulin secretion in the isolated pancreas fulfilling the definition of an incretin but it lowers insulin secretion in physiologically relevant conditions [6, 10, 18]

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Calcium, too, requires adequate levels of magnesium, vitamin K, and vitamin D to maintain healthy bones

Cells were seeded on Falcon dishes at 37 C with 5% CO 2 in Dulbeccos modified Eagle medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/ml penicillin (Beyotime, China) and 100 mg/ml streptomycin (Beyotime, China) [26]

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