Palmitic acid-DHA assay The fatty acids UC13-C16:0 and DHA were extracted from 40 l of cerebrovasculature by adding 10 volumes of isopropanol (IPA) and spiking samples with 5 l of 12 M d2-C16:0 (Cayman Chemicals) and 0.67 M d5-DHA (Cayman Chemicals) at the beginning of the extraction
C.PinedaC
Standard glutathione capsules and tablets are notorious for being poorly absorbed
Glutamate in cell medium was detected by using Amplex Red Glutamic Acid/Glutamate Oxidase Assay kit (# A-12221, Thermo Fisher) as per the manufacturers instructions
(5) A Nakamura, M Nakashima, T Sugao, H Kanemoto, et al (1988) Potent antinociceptive effect of centrally administered delta-sleep-inducing peptide (DSIP) European Journal Pharmacology, 1988 Oct 18, Volume 155 (Issue 3), Pages 247-53
For breast cancer, altered GPx1 expression influences prognosis, and targeting GPx4 could combat resistant cells via ferroptosis induction