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The study was conducted according to Helsinki principles, declared to the Commission Nationale de lInformatique et des Liberts (declaration number, 2067659) and approved by the ethics committee of the French Society of Intensive Care (protocol number, FICS20020231)

Hoechst staining Hoechst 33342 was used to specifically stain the nuclei of living and fixed cells and tissues, which allowed for the measurement of apoptosis within cells

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