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Also, prolonged NSW ( 10 years) correlated with hypomethylation in NR1D1, and hypermethylation of CSNK1E, a kinase regulating PER proteins downstream of CLOCK 215

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Twenty microliters of supernatant was transferred into a 96-well plate, and 200 l of 37 C o-dianisidine hydrochloride solution was added immediately before the optical density was read at 450 nm and again 30 s later, as previously described 26

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