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Medium from each of the four wells was collected and stored at 80 C prior to immunoassays

It undergoes enzymatic processing from its preproglucagon peptide precursor within intestinal L-cells, ultimately giving rise to GLP-1(1-37) and peptide variants including GLP-1(7-36)

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(6) Increased bone marrow adipose tissue accumulation and altered mesenchymal stem cell differentiation contribute to reduced osteoblast activity, increased osteoclastic resorption, and an overall decline in bone strength, leading to a greater fracture risk despite higher bone mineral density measurements