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Transfection HEK-293T cells were cultured in 100-mm dishes and transfected upon 70% confluency

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Each TCS typically comprises a membrane-bound histidine kinase (HK) and a cytoplasmic response regulator (RR), which together mediate signal transduction in response to environmental stimuli

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Similarly, the levels of four key sex hormones AMH, E2, FSH, and luteinizing hormone also displayed distinct patterns compared with the other models (Supplementary Fig