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In vitro experiments and in vivo studies employing animal models have demonstrated that SCFA supplementation elevates the transcriptional expression of TRPV6 and calbindin-D9k in both human colonic epithelial (Caco-2) cells and the colonic mucosa of rats (108, 185)

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Lipofectamine 3000 siRNA transfection reagent (Thermo Fisher Scientific, Carlsbad, CA, USA) was then used to transfect the cells with either negative control or Nrf2 siRNA duplexes at a concentration of 50 nM for 36 h