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Moving from 0.25 mg to 0.50 mg and from 0.50 mg to 1.0 mg represent the steepest relative dose jumps in the titration schedule (100% increases each time)

The rate constants from our deglutathionylation assays between 6.4 10 4 and 1.4 10 6 M 1 s 1 are very similar to the rate constants for PfGrx or plant and mammalian glutaredoxins from discontinuous or coupled enzymatic assays with other high and low molecular weight GSSR substrates (Table 1) 9,11,14,19 , thus confirming our assay as an alternative reliable method for the direct analysis of enzyme-catalyzed deglutathionylations

More recently we have used microarray profiling to demonstrate that the vast majority (78 %) of genes differentially expressed in wounds from elderly versus young men were estrogen-regulated [58]