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In brief, cells were imaged at the plasma membrane under vehicle conditions as well as following stimulation with 100 nM GLP-1 with an image size of 256256 pixels and 80 nm pixel size for 200 consecutive frames

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The following residues obtained a percentage equal to or greater than 80% in terms of hydrophobic contacts for several MD simulations, with the number of total appearances in parentheses: Trp-86 (9), Tyr-341 (9), Tyr-124 (8), Tyr-337 (8), Gly-121 (6), Trp-286 (6), Tyr-72 (6), Asp-74 (4), Gly-448 (4), Hid-447 (4), Met-85 (4), Phe-297 (4), Phe-338 (4), Thr-75 (4), Thr-83 (4), Glu-292 (3), Ser-125 (3), Val-73 (3), Asn-87 (2), Leu-76 (2), Phe-80 (2), Ser-293 (2), Tyr-449 (2), Tyr-77 (2), Val-294 (2), Ala-343 (1), Arg-296 (1), Gln-71 (1), Gly-122 (1), Gly-342 (1), Gly-362 (1), Gly-366 (1), Gly-82 (1), Ile-451 (1), Leu-339 (1), Leu-398 (1), Lys-348 (1), Phe-295 (1), Pro-344 (1), Ser-438 (1), Trp-439 (1), Tyr-133 (1), Val-132 (1), Val-365 (1)

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