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A 2018 review in the International Journal of Molecular Sciences analyzed available gene expression data and found that GHK-Cu at clinically relevant concentrations affected the expression of approximately 31.2% of human genes [2]

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Cells were harvested using 110 L ice cold lysis buffer containing 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl 2 , 1% (v/v) Triton X-100 and 10% (v/v) of protease and phosphatase inhibitor cocktail (Roche)

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