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The quantification methods include antioxidant assays (e.g., Glutathione assay, Ascorbic acid assay, Antioxidant enzyme activity assay), DNA damage/repair (e.g., 8-OH Guanine, double-strand break assay), peroxidation of lipids (e.g., 4-hydroxynonenal assay, malondialdehyde assay), ROS assay (e.g., H 2 O 2 assay, NO assay), protein modification (e.g., Advanced glycation end products, protein carbonyl assay), and Oxidase/Peroxidase activity (e.g., Monoamine oxidase assay, peroxidase/H 2 O 2 assay)

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doi: 10.1200/jco.2017.72.7735

This is largely due to: Limited Long-Term Data: Most studies have only focused on short-term use of DSIP

Clinical evidence demonstrates tirzepatide produces average weight loss of 20-22% over 68 weeks in phase 3 trials, whereas glutathione's weight loss mechanism remains primarily indirect through oxidative stress reduction