As mentioned previously, tumour/cancer cell metabolism is highly variable and is dependent on multiple factors like anatomical location, genetic factors, environmental conditions, lifestyle, pharmacological interventions, physiological conditions impacting the surrounding microenvironment, and availability of nutrients (illustrated in Fig
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indiscriminate use may mask the precise diagnosis
snRNA-seq and analysis For isolation of nuclei from gWAT, fresh adipose tissue was minced in nucleus preparation buffer (10 mM HEPES, 1.5 mM MgCl 2 , 10 mM KCl, 250 mM sucrose, 0.001% NP-40) with 0.2 U l Roche Protector RNase inhibitor followed by dounce homogenization
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