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Gao H, Zhao L, Wang H, Xie E, Wang X, Wu Q, et al

Unique neuronal functions of cathepsin L and cathepsin B in secretory vesicles: biosynthesis of peptides in neurotransmission and neurodegenerative disease

The third aliquot directly measures the combined pool of GSH and GSSG using previously published protocol with Ellmans reagent as a reporter

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They found that it wasnt possible to determine a clear lower limit for which any of these biomarkers can reliably indicate B12 deficiency, but that daily intakes above 4.0 g appear to have no additional benefit