The second BglII site at position 7107 in pcDNA3-GFP11(x7)-Actin was removed by site directed mutagenesis using Fwd 5- TGACGTCGACGGATCGGGAAATATCCCGATCCC-3 and Rev 5- GGGATCGGGATATTTCCCGATCCGTCGACGTCA-3 primers with the Quick-Change Lightning kit (Agilent) prior to in frame cloning of human VAPB using T4 DNA ligase (Promega)
Am J Clin Nutr 118, 369381.Google Scholar Baleztena, J, Ruiz-Canela, M, Sayon-Orea, C et al
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