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Assessment of Cell Viability ARPE-19 cell viability was evaluated by performing the 3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)2-(4-sulfophenyl)2 H-tetrazolium (MTS) assay as previously described [25]

Blum, R., Meyer, K

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Nat Rev Dis Primers 10(1):37

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Secondary endpoints were defined as pharmacodynamics and pharmacokinetic parameters including maximum serum concentration, time to maximum concentration, half-life, plasma clearance, and volume of distribution, as well as exploratory parameters including bodyweight, glycemic control, and overall hormone profile