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Effects should therefore be described strictly as observations within specific experimental systems rather than as established outcomes

( A ) Intracellular accumulation of cAMP was measured by enzymatic immunoassay in lysates from caSMCs treated for 10 minutes with 100 nM each of GLP-1, forskolin, or PBS control, with or without 20 M sacubitril, a neutral endopeptidase inhibitor ( n = 3/treatment, each in triplicate)

Sodium, potassium, and magnesium should be monitored and supplemented as needed

2002 Oct;48(5):325-31

To determine the optimal values for the sparsity parameters and the number of components (two) and features (four and five, respectively) 5-fold cross-validation (repeated ten times) was employed

Proc Natl Acad Sci 79(2):345349 Ma Y, Huang Y, Yan L, Gao M, Liu D (2013) Synthetic FXR agonist GW4064 prevents diet-induced hepatic steatosis and insulin resistance