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The solvent was removed in a speed vac (5 h, 30 C, Concentrator Plus, Eppendorf), and the resulting dried peptides were stored at 20 C until analysis, described in Binding site identification isoDTB analysis section in the Supplementary Information

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Specificity and inhibition studies of human renal dipeptidase

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These interventions can help reduce intestinal inflammation, promote the production of beneficial metabolites (e.g., SCFAs), and improve BBB integrity

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