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Phase 2 clinical trials involved 338 adults with obesity (BMI 30 or more) and overweight (BMI of 2729.99) with one or more weight-related conditions such as high blood pressure, high cholesterol levels, heart disease, and type 2 diabetes

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Labelled cells were then detached by trypsinization, pelleted, washed once with PBS, resuspended in 300 l ice-cold PBS and fixed by adding 700 l ice-cold absolute ethanol while gently vortexing

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