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Measurements All measurements were conducted on a PerkinElmer NexION 5000 inductively coupled plasmatriple quadrupole mass spectrometer under dynamic reaction cell (DRC) mode at the London Metallomics Facility, Kings College London

For experiments FOXO4-DRI was dissolved in DMEM to generate a 2.5 mM stock solution

Vitamin B1 (Thiamine): Helps convert carbohydrates and fats into energy

As the most pharmacologically complex compound in the Velox Peptides catalogue, Retatrutide is primarily of interest to researchers studying multi-receptor metabolic signalling, incretin biology, and energy homeostasis

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