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Grether-Beck S, Marini A, Jaenicke T, Krutmann J

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The factors that lead to depletion of glutathione includes natural ageing process, or the external stressors that include chronic exposure to chemical toxins, cadmium exposure, alcohol use, smoking, pollution, strenuous aerobic activities, dietary factors, ultraviolet (UV) radiation exposure, tissue injury as from burns, ischemia, septic shock, surgery, drugs (such as acetaminophen and adriamycin)

Detection of ROS level Fresh ovarian tissue was placed in ROS staining solution (Beyotime, S0033S, Shanghai, China) and stained in an incubator at 37 C for 25 min