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However, current evidence remains largely proof-of-concept, and translation requires careful evaluation of phototoxicity, cross-platform calibration, and protocol-dependent domain shift (culture media, incubator conditions, optics), so hyperspectral features are best positioned within standardized, prospectively validated multimodal pipelines rather than as stand-alone add-ons [444, 445]

* Correspondence: Suhua Guan, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

It is, however, difficult to retain participants and increasing the number of measurements in large samples becomes cost prohibitive

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Medicine 2021;100:e26427 9