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Microrna-27a-3p affects estradiol and androgen imbalance by targeting Creb1 in granulosa cells in a mouse polycystic ovary syndrome model

After stripping away the bulb from the HFs, the remaining hair follicles were collected and placed directly in DMEM/F12 medium containing 10% FBS and 100 mg/mL penicillin/streptomycin, followed by undisturbed culture under normal conditions for 10 days