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Further structural optimization delivered a large number of macrocyclic disulfides and among them 23 (HA08) encompassing a 3-homotyrosine residue in a 13-membered ring system and exhibiting the lowest Ki value in the series (Ki = 3.3 nM)

The enzyme resistance results in a distinct metabolic pathway of exendin-4

"No pain, no gain" loses its motivating ability when it tends to be all pain and very little gain, for weeks on end, doesn't it

Li, Zhang 2010

Here is an overview of a few promising options

To confirm that these proteins were susceptible to glutathionylation, we treated the recombinant protein with BioGEE in vitro and analysed the extent of BioGEE labelling by Western blot using streptavidin-HRP