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We show (i) that PfGrx-catalyzed redox reactions outcompete uncatalyzed reactions between roGFP2 and GSH or GSSG by several orders of magnitude, (ii) that the PfGrx-catalyzed reduction and oxidation of roGFP2 occur via a mono- and not a dithiol mechanism, and (iii) that the reduction of roGFP2(S 2 ) as a model non-glutathione disulfide substrate most likely involves a rate-limiting glutathionylation in a ternary complex with a GSH molecule that is activated at the 1st glutathione-interaction site of PfGrx
The practical reality involves individual use occurring under this research designation, though such use technically falls outside intended purposes
Pipette 5 mL of the above solution into a 100 mL conical flask, add another 5 mL of 2% HPO solution to make a total volume of 10 mL, then add 1 mL of 5% potassium iodide and 2 drops of starch indicator
