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A reduction in CYPOR protein levels prevented NFT-dependent accumulation of Nrf2 protein and partially attenuated the NFT-dependent increase in glutathione levels

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Reliable quantification of individual NAD metabolites and glutathione can be achieved through liquid-chromatography mass-spectrometry (LC-MS) 22 , cyclic enzymatic assays and high-performance liquid chromatography (HPLC) 2326 , but these have not been applicable for the scalable analysis of the whole redox set, especially from a single extract

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To evaluate the impact of uridine supplementation on cytoplasmic maturation, we analyzed the dynamics of cortical granules (CGs) and their contents ovastacin, which are vital for blocking polyspermy in oocytes 28,29