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While Ghai maintained these drips were "not unsafe," her family conceded that Jariwala consumed these supplements and over-the-counter pills without a doctor's direct supervision Mumbai: The sudden passing of actor Shefali Jariwala at age 42, on June 27, has cast a spotlight on her final hours, with police inquiries focusing on a combination of a religious fast and an unsupervised intravenous (IV) drip as potential factors

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DNA and transfection reagent was diluted in 250 L Opti-MEM (Gibco) separately, mixed and incubated for 10 min at room temperature

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