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Other authors, using co-culture of CD4 + T cells, demonstrated a similar effect on FLS, suggesting that the secretion of proinflammatory products of CD4 + T can promote glycolysis in FLS while downregulating OXPHOS 41

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We also used alkaline phosphatase, a disulfide-dependent enzyme that had been engineered not to be secreted, to detect thiol oxidation events inside the cytoplasm