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Clinically, multiple cohort studies support a causal link between vitamin D deficiency and AKI risk

The vitamin has low toxicity, so supplementation carries minimal risk, but also likely provides no benefit if stores are adequate

Western blot Cells were harvested and proteins were extracted using cell lysis buffer supplemented with 0.3% phenylmethylsulfonyl fluoride and proteinase and phosphatase inhibitors

This is the safe, conservative number that applies to the majority of common research peptides

doi: 10.3390/molecules24030449, 114

With continued medication use, spontaneous resolution has been documented at four to six weeks and up to four months