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5 4) Tendon & connective-tissue pathways (GHR up-regulation) In tendon fibroblasts, BPC-157 up-regulates the growth-hormone receptor (GHR) at mRNA and protein levels and augments downstream JAK2 signaling in response to GHmechanisms consistent with tendon repair biology (in-vitro)

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Then, the cells were subjected to immunofluorescence staining for -SMA, desmin, GFAP, and vimentin (Supplementary Table 2) by an overnight incubation with primary antibodies at 4 C, followed by a 1-h incubation with secondary antibody (1:500, Molecular Probes) after a wash step

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Route note: 5-Amino-1MQ is well documented as orally bioavailable and is typically sold and dosed as an oral capsule