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The gradient elution program was optimized as follows: 5% phase B at 01.00 min

Barcoded universal forward 515F primers and 806R reverse primers containing Illumina adapter sequences, which target the highly conserved V4 region, were used to amplify microbial DNA ( 16S rRNA gene sequencing produced 2,610,204 reads with a median of 50,609 per sample (range: 9,512 470,848)

Recently, the bacterial IMPDH, GuaB (inosine-5-monophosphate dehydrogenase) enzyme, a compound of the purine nucleotide biosynthetic pathway, has become popular as a potential target for eradication multi-drug resistant pathogens such as Cryptosporidium parvum or Mycobacterium tuberculosis

(C) Plate assay of A