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The sections were sealed for 1 h with 10% goat serum before being incubated with rabbit anti-GZMB (1:500, 13588-1-AP, ProteinTech Group, RRID: AB_2114429), mouse anti-CD8A (1:200, 66868-1-Ig, ProteinTech Group, RRID: AB_2882205), and mouse anti-CD56 (1:200, 60238-1-Ig, ProteinTech Group, RRID: AB_2881361) overnight at 4 C

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The HOMA-IR significantly increased in the Met+Sita group (1.662.33, P =0.024), but not in the Met+Sita+Acarb group (1.202.80, P =0.092) at week 16 versus baseline (Supplementary Table 3)

Moreover, NADPH binding induced local conformational changes in the N-terminal long loop (residues 3459) of Pa DHDPR, as the His35 and Lys36 residues in this loop interacted with the 2-phosphate group of NADPH, possibly accounting for the strong preference of Pa DHDPR for NADPH

2010;92(3):574-584 Jarlenski MP, Bennett WL, Bleich SN, Barry CL, Stuart EA