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Since these C-capsids (125 nm) are too large to pass through nuclear pores, they reach the cytoplasm by an unconventional route 30 that first involves their budding through the inner nuclear membrane, which produces enveloped perinuclear virions

Tilting the vial and positioning the needle tip in the deepest point helps access this remaining volume

Reduction capacity assays FRAP (ferric reducing antioxidant power) assay The FRAP assay is a single electron transfer-based method widely used for evaluating the antioxidant capacity of various samples, including plant extracts, foods, and biological fluids (Benzie & Strain, 1996

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both walk through the next steps clearly