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In adults, capillary endothelial cells (ECs) are the main component of the BBB and constitute a blood-brain exchange interface area of 12 to 18 cm 2

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Cell culture medium was removed and Tag-lite labeling medium with 100 nM of SNAP-Lumi4-Tb (Cisbio, SSNPTBC) was added, and the cells were further incubated for 1 h at 37 C in 5% CO 2

You can also use ice but wrapped in cloth, not applied directly to the skin