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After the addition of a formate buffer and centrifugation, the supernatant fluid was subject to solid-phase extraction with the addition of F 2 -isoprostane-d4 as an internal standard by using an Oasis HLB extraction cartridge preconditioned with methanol and 10 mmol formate buffer/L (pH 3.0) for isoprostane extraction

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The treatment currently relies largely on weekly injections, though pill formats are expected in future, which may widen participation further

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