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JA and JA-Ile quantification in leaf tissues Metabolite extraction and quantification of JA and JA-Ile in leaf tissues were performed according to previously described methods using AB Sciex 4500 QTRAP triple quadrupole mass spectrometer (AB SCIEX, MA, USA) equipped with an ACQUITY UPLC BEH C18 column (Waters, Eschborn, Germany) (50 2.1 mm, 1.7 m) 45

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30 cycles of 98 C (10 s), 50 C (30 s), 72 C (30 s)

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Acupuncture: This traditional Chinese medicine technique may help balance the bodys energy flow (Qi) and has been found to be beneficial in managing stress and hormonal imbalances

To further functionalize the nanoparticles, 20 mg of glutathione (GSH) was added to the solution