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The beads were washed three times with 1 ml of high-salt buffer (20 mM HEPES-KOH (pH 7.9), 500 mM KCl, 0.2 % NP-40, 15 % glycerol, 1 mM DTT) with rotation for 5 min at 4 C during each wash, followed by one detergent-free wash (20 mM HEPES-KOH (pH 7.9), 150 mM KCl, 15% glycerol, 1 mM DTT)

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Most side effects are dose dependent and can be transient

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Biofilms were then washed once with 1 PBS and thoroughly homogenized by pipetting, with 200 L of 1 PBS

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