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The His tag of the purified protein was removed by thrombin treatment, and the protein was applied to a Superdex 200 column (GE Healthcare, Piscataway, NJ, USA) pre-equilibrated with buffer comprising 50 mM Tris-HCl (pH 8.0) and 150 mM NaCl

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Among those, 432 helicoid III nanoparticles particularly exhibited well-defined chiral morphologies and exceptional chiroptic properties, evidenced by a Kuhns dissymmetry factor (g-factor) of 0.2, making them valuable for various applications

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The Human Tripeptide GHK and Tissue Remodeling. Journal of Biomaterials Science U.S