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Effect of TMAO on macrophage cholesterol biosynthesis, cholesterol efflux, inflammatory genes, and desmosterol levels The effect of cholesterol loading on macrophage cholesterol biosynthetic and inflammation genes, LDL receptor expression levels, and desmosterol levels, were performed as previously described 24
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CryoEM data processing All data were processed using the Cryo-EM Single Particle Ab-Initio Reconstruction and Classification (cryoSPARC) software suite 40
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The tissue sections were stained with Safranin O-Fast Green (SOFG), Hematoxylin & Eosin (H&E) and Sirius Red
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