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The supernatants were collected for further protein quantification using BCA protein assay kit after centrifugation at 12,000 g at 4C for 15 minutes

10.1073/pnas.0808180106 Proc

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This may be due to nuclear or cytoplasmic disturbances manifested as cytoplasmic vacuolization and fragmentation, a large perivitelline space, spindle disturbances, or mitochondrial aggregation [36]

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