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Methods Antibodies Anti-MBP tag mouse monoclonal antibody and anti c-Jun rabbit monoclonal antibody was used at 1:1000 dilution (Beyotime, AF2912, AF1612), anti-ubiquitin was used at 1:20000 dilution (Abcam, ab134953), anti-GST tag mouse monoclonal antibody was used at 1:5000 dilution (Yeason, 30901ES50), HRP-conjuagated FLAG-tag mouse monoclonal antibody was used at 1:1000 dilution (Yeason, 30502ES60)

Informed consent, documentation, and liability exposure for adverse outcomes all carry forward unchanged

The sections were deparaffinized in xylene and rehydrated through a graded ethanol series

Combination peptide protocols require careful planning

More serious complications like infection or allergic reactions occur in less than 1% of properly administered injections

Where recent U.S