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Injection technique after reconstitution Reconstituting the peptide correctly is only half the equation

cytokine-mediated cell death

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In addition to the cell culture media described above, strial explants can also be maintained in the following media: MEM with Earle's salts, 15 mM HEPES buffer, 2 mM L-glutamine, 10 mg/L MEM non-essential amino acids, 2.5 mg/L fungizone, 100 units/ml penicillin, 100 mg/L streptomycin, 400 g/L hydrocortisone, 5 mg/L transferrin, 2 nM triiodothyronine, 5 mg/L insulin, 100 M cholera toxin, 10 g/L epidermal growth factor, and 10% fetal calf serum