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In the second scenario, the final yield of your elution is close to 100 mg of GST-fusion protein, so youre feeling confident that you used the right binding buffer and that your protein isnt too big

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Certificate of Analysis Certificates of Analysis Janoshik Third-party tested Independent analytical lab Frequently Asked Questions IGF-1 LR3 is a modified 83-amino acid analog of IGF-1 with reduced binding to IGF binding proteins, resulting in greater bioavailability and a longer functional half-life than native IGF-1

Scand J Public Health